ssc buffer rnase free (Thermo Fisher)

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Thermo Fisher ssc buffer rnase free
Ssc Buffer Rnase Free, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/SSC+buffer/pmc13293986-80-1-4
Average 97 stars, based on 1 article reviews

ssc buffer rnase free - by Bioz Stars, 2026-09

97/100 stars

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Hybridization:

Article Title: lncRNA AL445238.2‑USP4 axis regulates cell survival and stemness in colon cancer.
Article Snippet: For FISH staining of AL445238.2, after permeabilization, cells were incubated with pre‐hybridization buffer (Abnova Corporation; cat. no. U0028) at 37 ̊C for 30 min, then hybrid‐ ized with Alexa Fluor 555‐labeled FISH probe provided by GenePharma Co., Ltd. at 37 ̊C overnight in the dark. .. Following hybridization, cells were washed with SSC buffer (Thermo Fisher Scientific, Inc.; cat. no. J60839.K2; 2X SSC for 5 min, 1X SSC for 5 min and 0.5X SSC for 5 min) at 37 ̊C. ..

Article Title: lncRNA AL445238.2 -USP4 axis regulates cell survival and stemness in colon cancer
Article Snippet: For FISH staining of AL445238.2 , after permeabilization, cells were incubated with pre-hybridization buffer (Abnova Corporation; cat. no. U0028) at 37°C for 30 min, then hybridized with Alexa Fluor 555-labeled FISH probe provided by GenePharma Co., Ltd. at 37°C overnight in the dark. .. Following hybridization, cells were washed with SSC buffer (Thermo Fisher Scientific, Inc.; cat. no. J60839.K2; 2X SSC for 5 min, 1X SSC for 5 min and 0.5X SSC for 5 min) at 37°C. ..

Fluorescence In Situ Hybridization:

Article Title: Direct targeting of C9ORF72 repeat RNA with fluorinated antisense oligonucleotides.
Article Snippet: .. On the day of the FISH staining, cells were rinsed for 2 min in 2 × SSC buffer (Thermo Fisher Scientific) with 15% formamide. ..

Staining:

Incubation:

Article Title: Single-cell Stereo-seq reveals regulatory mechanisms driving regeneration of injured proximal tubules during AKI.
Article Snippet: .. After washed with 0.1× SSC buffer (Thermo, AM9770) supplemented with 0.05 U/mL RNase inhibitor (NEB, M0314L), tissue sections placed on the chip were permeabilized using 0.1% pepsin (Sigma, P7000) in 0.01 M HCl buffer, incubated at 37°C for 5 minutes and then washed with 0.1×SSC buffer (Thermo, AM9770) supplemented with 0.05 U/mL RNase inhibitor (NEB, M0314L). .. RNA released from the permeabilized tissue and captured by the DNB was reverse transcribed 2h at 42°C using SuperScript II (Invitrogen, 18064-014, 10 U/mL reverse transcriptase, 1 mM dNTPs, 1 M betaine solution PCR reagent, 7.5 mM MgCl2, 5 mM DTT, 2 U/mL RNase inhibitor, 2.5 mM Stereo-seq-TSO and 1×First-Strand buffer).

Article Title: Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling
Article Snippet: Total RNA was isolated using the Quick-RNA Miniprep Kit (Zymo Research) according to the manufacturer’s instructions and quantified by NanoDrop spectrophotometry. .. For digestion controls, 5 μg of RNA was incubated in 50 μL of 1 × SSC buffer at 37 °C for 30 min with either 1 μL RNase A/T1 mix (2 mg/mL RNase A, 5000 μ/mL RNase T1; Thermo Fisher Scientific) + 1 μL RNase I (100 μ/μL; Thermo Fisher Scientific), or 1 μL Proteinase K (20 mg/mL). .. Digested samples and untreated controls were purified twice using the Monarch RNA Cleanup Kit (New England Biolabs) according to the manufacturer’s instructions.

Magnetic Beads:

Article Title: On-site amplification-free electrochemical detection of the plant pathogen Xylella fastidiosa via cathodic potential-induced DNA adsorption.
Article Snippet: Plant diseases pose a growing threat to global food security, with invasive bacterial pathogens presenting particular challenges for early detection and containment.. Xylella fastidiosa is among the most destructive of these pathogens, infecting hundreds of plant species and posing a severe biosecurity risk to agricultural systems, including those in Australia.. Despite its significance, routine detection still relies on laboratorybased molecular amplification methods that are slow, costly, and poorly suited to field deployment.

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